Dot immunoperoxidase assay for detection of parvovirus B19 antigens in serum samples
نویسندگان
چکیده
منابع مشابه
Dot immunoperoxidase assay for detection of parvovirus B19 antigens in serum samples.
We describe a simple and rapid dot immunoperoxidase assay for the direct detection of parvovirus B19 capsid antigens in human sera. The assay was performed with serum specimens dotted onto nylon membranes. VP1 and VP2 B19 antigens, which represent 4 and 96% of the capsid, respectively, were detected with a pool of monoclonal antibodies directed against the two proteins, and the complex was visu...
متن کاملUndenatured parvovirus B19 antigens are essential for the accurate detection of parvovirus B19 IgG.
Recombinant versions of parvovirus B19 capsid proteins VP1 and VP2 are used for immunodiagnostic assays for detection of antiviral antibodies. The immune response to B19 is characterized by a gradual loss of antibodies directed against linear epitopes of VP2. A similar occurrence for antibodies raised against VP1 protein would represent a limitation to serological assays incorporating denatured...
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A chemiluminescence dot blot hybridization assay was used for the detection of B19 parvovirus DNA in human sera by using digoxigenin-labeled probes. The probes were revealed immunoenzymatically by use of anti-digoxigenin Fab fragments conjugated with alkaline phosphatase. The chemiluminescence signal was obtained by reacting the labeled probe-target complex with an enzyme-triggerable dioxetane ...
متن کاملDot immunoperoxidase assay using monoclonal antibody for direct detection of cytomegalovirus in urine samples.
A rapid, simple dot immunoperoxidase assay for the direct detection of cytomegalovirus in clinical urine samples was developed. The assay was performed on nitrocellulose paper dotted with urine pellets free of cellular debris. Cytomegalovirus was detected with a monoclonal antibody to the capsid antigen, and the complex was visualized by immunoperoxidase staining. Positive reactions appeared as...
متن کاملDetection of Human Parvovirus B19 Markers in Blood Samples of Donors
Background and Aims: Human parvovirus B19, a member of the parvoviridae family, with single-stranded DNA is a very minute non-enveloped virus. B 19 virus is mostly transmitted via the respiratory tract but some studies have been reported which B19 virus can be transmitted through blood and/or blood products. The aim of this study was to evaluate the prevalence of B19 among blood donors in Tehra...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1997
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.35.6.1575-1578.1997